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dc.contributor.authorSoydan, S. S.
dc.contributor.authorAraz, K.
dc.contributor.authorSenel, F. V.
dc.contributor.authorYurtcu, E.
dc.contributor.authorHelvacioglu, F.
dc.contributor.authorDagdeviren, A.
dc.contributor.authorTekindal, M. A.
dc.contributor.authorSahin, F.
dc.date.accessioned2023-12-13T11:33:38Z
dc.date.available2023-12-13T11:33:38Z
dc.date.issued2015
dc.identifier.issn0960-3271en_US
dc.identifier.urihttp://hdl.handle.net/11727/11086
dc.description.abstractData arising from the recent literature directed the researchers to study on the degree and extent of bisphosphonate toxicity on oral mucosa in further detail. The aim of this study is to determine the half maximal inhibitory concentration of pamidronate (PAM) and alendronate (ALN) on human gingival fibroblasts in vitro using 3-[4.5-thiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT) assay and to evaluate the effects of both agents on the proliferation and apoptotic indices. Cells used in the study were generated from human gingival specimens and divided into alendronate (n = 240), PAM (n = 240), and control groups (n = 60). Based on the MTT assay results, 10(-4), 10(-5), 10(-6), and 10(-7) M concentrations of both drugs were administered and the effects were evaluated for 6, 12, 24, 48, or 72 h periods. An indirect immunofluorescence technique was used to evaluate apoptotic (anti-caspase 3) and proliferation (anti-Ki67) indices. Toxicity of both PAM and ALN was found to be the most potent at 10(-4)-10(-5) M range. The apoptotic index of PAM group was found to be significantly higher than ALN group for all concentrations especially at 24 h incubation time (p < 0.05). The decrease in the proliferation index was found similar in first 48 h for both drugs; however, after 72 h of incubation decrease in proliferation index in PAM group was found to be significantly higher (p < 0.05). Micromolar concentrations of not only PAM but also ALN rapidly affect cells generated from human oral gingival tissue by inducing apoptosis together with inhibition of proliferation. Cytotoxic effects of both ALN and PAM on primary human gingival fibroblasts, which cause significant changes in apoptotic and proliferative indices as shown in this in vitro study, suggests that the defective epithelialization of oral mucosa is possibly a major factor on the onset of bisphosphonate-related osteonecrosis of the jaw cases.en_US
dc.language.isoengen_US
dc.relation.isversionof10.1177/0960327115569808en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBisphosphonate-related osteonecrosis of the jawen_US
dc.subjectalendronateen_US
dc.subjectoral mucosaen_US
dc.subjectfibroblasten_US
dc.subjectapoptosisen_US
dc.subjectcell deathen_US
dc.subjecttoxicity testingen_US
dc.titleEffects of Alendronate and Pamidronate on Apoptosis and Cell Proliferation in Cultured Primary Human Gingival Fibroblastsen_US
dc.typearticleen_US
dc.relation.journalHUMAN & EXPERIMENTAL TOXICOLOGYen_US
dc.identifier.volume34en_US
dc.identifier.issue11en_US
dc.identifier.startpage1073en_US
dc.identifier.endpage1082en_US
dc.identifier.wos000363214300004en_US
dc.identifier.scopus2-s2.0-84944071051en_US
dc.identifier.eissn1477-0903en_US
dc.contributor.pubmedID25636638en_US
dc.contributor.orcID0000-0002-6026-0045en_US
dc.contributor.orcID0000-0001-8990-8282en_US
dc.contributor.orcID0000-0001-7308-9673en_US
dc.contributor.orcID0000-0003-4930-8164en_US
dc.contributor.orcID0000-0002-4060-7048en_US
dc.contributor.orcID0000-0002-4475-0861en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergien_US
dc.contributor.researcherIDAAH-8887-2021en_US
dc.contributor.researcherIDP-2877-2014en_US
dc.contributor.researcherIDAAC-7232-2020en_US
dc.contributor.researcherIDAAA-2998-2021en_US
dc.contributor.researcherIDAES-7155-2022en_US
dc.contributor.researcherIDU-9270-2018en_US


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