dc.contributor.author | Kozanoglu, Ilknur | |
dc.contributor.author | Iplik, Elif Sinem | |
dc.contributor.author | Ertugrul, Baris | |
dc.contributor.author | Baran, Yusuf | |
dc.contributor.author | Cakmakoglu, Bedia | |
dc.date.accessioned | 2019-05-08T12:36:21Z | |
dc.date.available | 2019-05-08T12:36:21Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 2008-3866 | |
dc.identifier.uri | http://ijbms.mums.ac.ir/article_10541.html | |
dc.identifier.uri | http://hdl.handle.net/11727/3246 | |
dc.description.abstract | Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.
Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.
Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells.
Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity. | en_US |
dc.language.iso | eng | en_US |
dc.relation.isversionof | 10.22038/IJBMS.2018.26152.6420 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Colon cancer | en_US |
dc.subject | Stem cells | en_US |
dc.subject | Cell death | en_US |
dc.title | An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue | en_US |
dc.type | article | en_US |
dc.relation.journal | IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES | en_US |
dc.identifier.volume | 21 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.startpage | 465 | en_US |
dc.identifier.endpage | 468 | en_US |
dc.identifier.wos | 000433360100004 | en_US |
dc.identifier.scopus | 2-s2.0-85045298758 | en_US |
dc.contributor.pubmedID | 29922425 | en_US |
dc.contributor.orcID | 0000-0002-5268-1210 | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | en_US |
dc.contributor.researcherID | AAE-1241-2021 | en_US |