Show simple item record

dc.contributor.authorErsozlu, S.
dc.contributor.authorSarisozen, B.
dc.contributor.authorOzer, O.
dc.contributor.authorAdim, S.B.
dc.contributor.authorSahin, O.
dc.date.accessioned2019-06-30T20:34:55Z
dc.date.available2019-06-30T20:34:55Z
dc.date.issued2017
dc.identifier.issn21971153
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519512/
dc.identifier.urihttp://hdl.handle.net/11727/3764
dc.description.abstractBackground: Although, glucocorticoid (GC) and calcitonin-induced changes in bone repair have been studied previously, the exact effects of these on fracture healing remain controversial. Hence, the purpose of this experimental study is to determine biochemical and histological effects of locally administrated GC and systemically administrated calcitonin on the kinetics of healing response after bone marrow ablation in rats. Methods: After having undergone marrow ablation, a steroid-treated group of rats (n = 24) received a single dose of intramedullary methylprednisolone (2 mg/kg), a calcitonin-treated group (n = 24) received intermittently administrated subcutaneous salmon calcitonin (16 IU/kg), and a control group (n = 24) received intramedullary saline (25 μl). Results: Blood samples taken on days 1, 3, 7, 9, and 15 after ablation showed an increase in serum calcium, alkaline phosphatase (ALP), and phosphate levels in the Calcitonin and Control groups. Levels of calcium and ALP peaked on day 7 after ablation. However, an increase in phosphate levels indicated a biphasic reaction that peaked on the third and ninth day after ablation. Hypercalcemia was not observed in Steroid group because of the inhibition of osteoclastic bone resorption. In that group, the serum levels of ALP and phosphate were lower than baseline levels. The levels of urinary calcium excretion peaked 3 to 7 days after marrow ablation in the control group and 7 to 9 days after that procedure in the steroid group. Histologic evaluation showed that the rats in the control group demonstrated the expected healing period according to the histological grades and that a delay in healing occurred in the calcitonin group after day 9 because of the inhibition of osteoclastic bone resorption. All rats in the steroid group exhibited a decrease and delayed healing response. Conclusion: Total serum calcium, phosphate, and ALP levels increased after bilateral tibial bone marrow ablation and urine calcium and hydroxyproline excretion also increased as a factor of bone resorption. Subcutaneously administrated salmon calcitonin did not affect biochemical changes after marrow ablation. Single-dose intramedullary methylprednisolone inhibited extra-tibial bone resorption induced by cytokines after bone marrow ablation. © 2017, The Author(s).en_US
dc.language.isoengen_US
dc.relation.isversionof10.1186/s40634-017-0100-xen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBone repairen_US
dc.subjectCalcitoninen_US
dc.subjectFracture healingen_US
dc.subjectMarrow ablationen_US
dc.subjectMethylprednisoloneen_US
dc.titleThe biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in ratsen_US
dc.typearticleen_US
dc.relation.journalJournal of Experimental Orthopaedicsen_US
dc.identifier.volume4en_US
dc.identifier.issue1en_US
dc.identifier.scopus2017-3


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record