• Türkçe
    • English
  • English 
    • Türkçe
    • English
  • Login
View Item 
  •   DSpace Home
  • Fakülteler / Faculties
  • Diş Hekimliği Fakültesi / Faculty of Dentistry
  • View Item
  •   DSpace Home
  • Fakülteler / Faculties
  • Diş Hekimliği Fakültesi / Faculty of Dentistry
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methods

Thumbnail
Date
2019
Author
Kayar, Nezahat Arzu
Oduncuoglu, Bahar Fusun
Haliloglu, Seyfullah
Serpek, Behic
Ataoglu, Tamer
Alptekin, Nilgun Ozlem
Metadata
Show full item record
Abstract
Objectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods. Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Results: NE activity (mu U) and NE activity/volume (mu U/mu l) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05). Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.
URI
http://hdl.handle.net/11727/4816
Collections
  • Diş Hekimliği Fakültesi / Faculty of Dentistry [72]
  • PubMed İndeksli Yayın Koleksiyonu [373]
  • Scopus İndeksli Yayın Koleksiyonu [450]
  • Wos İndeksli Yayınlar Koleksiyonu [745]

Related items

Showing items related by title, author, creator and subject.

  • Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methods 

    Kayar, Nezahat Arzu; Oduncuoglu, Bahar Fusun; Halilolgu, Seyfullah; Serpek, Behic; Ataoglu, Tamer; Alptekin, Nilgun Ozlem (2019)
    Objectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the ...
  • Kinetic Study on the OSL Technique Using Human Blood Sample 

    Koc, Kemal; Tanir, Gunes; Aksoy, Gulper; Bolukdemir, M. Hicabi (2018)
    It is dear that humans are exposed to ionizing radiation both of internally or externally in radiotherapy. The determination of ionizing radiation dose in human blood has been previously performed by us using optically ...
  • A Retrospective Evaluation of the Epithelial Changes/Lesions and Neoplasms of the Gallbladder in Turkey and a Review of the Existing Sampling Methods: A Multicentre Study 

    Ozgun, Gonca; Esendaglı, Guldal; Akarca, F. Goknur; Balci, Serdar; Argon, Asuman; Sengiz Erhan, Selma; Turhan, Nesrin; Ince Zengin, Neslihan; Hallac Keser, Sevinc; Celik, Betul; Bulut, Tangul; Abdullazade, Samir; Erden, Esra; Savas, Berna; Bostan, Temmuz; Sagol, Ozgul; Aysal Agalar, Anil; Kepil, Nuray; Karslioglu, Yildirim; Gunal, Armagan; Markoc, Fatma; Saka, Burcu; Ozdamar, Sukru Oguz; Bahadir, Burak; Kaymaz, Esin; Isik, Emre; Ayhan, Semin; Tuncel, Deniz; Ozguven Yilmaz, Banu; Celik, Sevinc; Karabacak, Tuba; Erbarut Seven, Ipek; Ataizi Celikel, Cigdem; Gucin, Zuhal; Ekinci, Ozgur; Akyol, Gulen (2018)
    Objective: As there is continuing disagreement among the observers on the differential diagnosis between the epithelial changes/lesions and neoplasms of the gallbladder, this multicentre study was planned in order to assess ...

DSpace software copyright © 2002-2016  DuraSpace
Contact Us | Send Feedback
Theme by 
Atmire NV
 

 

  • Politika

Politika
Kullanıcı Rehberi
Başkent Üniversitesi Kütüphanesi
Başkent Üniversitesi

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsxmlui.ArtifactBrowser.Navigation.browse_typexmlui.ArtifactBrowser.Navigation.browse_languagexmlui.ArtifactBrowser.Navigation.browse_publicationcategoryThis CollectionBy Issue DateAuthorsTitlesSubjectsxmlui.ArtifactBrowser.Navigation.browse_typexmlui.ArtifactBrowser.Navigation.browse_languagexmlui.ArtifactBrowser.Navigation.browse_publicationcategory

My Account

LoginRegister

Statistics

View Usage Statistics

DSpace software copyright © 2002-2016  DuraSpace
Contact Us | Send Feedback
Theme by 
Atmire NV