Show simple item record

dc.contributor.authorBaskin, Esra
dc.contributor.authorAykanat, Banu
dc.contributor.authorDemircigil, Gonca Cakmak
dc.contributor.authorBuyan, Necla
dc.contributor.authorGulleroglu, Kaan
dc.contributor.authorFidan, Kibriya
dc.contributor.authorBayrakci, Umut Selda
dc.contributor.authorDalgic, Aydin
dc.contributor.authorKarakayali, Hamdi
dc.contributor.authorHaberal, Mehmet
dc.contributor.authorBurgaz, Sema
dc.date.accessioned2019-06-18T12:40:24Z
dc.date.available2019-06-18T12:40:24Z
dc.date.issued2016
dc.identifier.issn0004-1254
dc.identifier.urihttps://www.degruyter.com/downloadpdf/j/aiht.2016.67.issue-4/aiht-2016-67-2851/aiht-2016-67-2851.pdf
dc.identifier.urihttp://hdl.handle.net/11727/3596
dc.description.abstractThe objective of this study was to reveal the likely genomic instability in children with chronic kidney disease (CKD) using micronucleus (MN) assay on buccal epithelial cells (BEC). We investigated the frequencies of micronuclei and other nuclear anomalies, such as nuclear buds, binucleated cells, condensed chromatin, and karyorrhectic and pyknotic cells in BEC. Children with CKD were grouped as follows: children in the pre-dialysis (PreD) stage (N=17), children on regular haemodialysis (HD) (N=14), and children who have undergone transplantation (Tx) (N=17). As a control group, twenty age-and gender-matched healthy children were selected. The MN frequency in BEC of all groups of children with CKD was significantly elevated (5-to 7-fold) as compared to the control group (p<0.001). In contrast, the frequencies of nuclear buds were not significantly higher in the study groups compared to the control group. The frequencies of binucleated cells and condensed chromatin cells were significantly higher in all subgroups of children with CKD relative to the control group (p<0.001). Our results show that the BEC of pediatric PreD, HD, and Tx patients with CKD display increased cytogenetic, cytokinetic, and cytotoxic effects. They also point to the sensitivity and usefulness of the BEC MN assay in the assessment of genetic susceptibility of patients with CKD.en_US
dc.language.isoengen_US
dc.relation.isversionof10.1515/aiht-2016-67-2851en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBuccal micronucleus assayen_US
dc.subjectGenotoxicityen_US
dc.subjectDialysisen_US
dc.subjectRenal transplantationen_US
dc.subjectNuclear anomaliesen_US
dc.titleMicronuclei and other nuclear anomalies in buccal epithelial cells of children with chronic kidney diseaseen_US
dc.typearticleen_US
dc.relation.journalArhiv za Higijenu Rada i Toksikologiju-Archives of Industrial Hygiene and Toxicologyen_US
dc.identifier.volume67en_US
dc.identifier.issue4en_US
dc.identifier.startpage317en_US
dc.identifier.endpage325en_US
dc.identifier.wos000393020600009


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record