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dc.contributor.authorAsghar, Waseem
dc.contributor.authorYuksekkaya, Mehmet
dc.contributor.authorShafiee, Hadi
dc.contributor.authorZhang, Michael
dc.contributor.authorOzen, Mehmet O.
dc.contributor.authorInci, Fatih
dc.contributor.authorKocakulak, Mustafa
dc.contributor.authorDemirci, Utkan
dc.date.accessioned2019-09-18T11:20:42Z
dc.date.available2019-09-18T11:20:42Z
dc.date.issued2016
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.nature.com/articles/srep21163.pdf
dc.identifier.urihttp://hdl.handle.net/11727/3954
dc.description.abstractAlthough materials and engineered surfaces are broadly utilized in creating assays and devices with wide applications in diagnostics, preservation of these immuno-functionalized surfaces on microfluidic devices remains a significant challenge to create reliable repeatable assays that would facilitate patient care in resource-constrained settings at the point-of-care (POC), where reliable electricity and refrigeration are lacking. To address this challenge, we present an innovative approach to stabilize surfaces on-chip with multiple layers of immunochemistry. The functionality of microfluidic devices using the presented method is evaluated at room temperature for up to 6-month shelf life. We integrated the preserved microfluidic devices with a lensless complementary metal oxide semiconductor (CMOS) imaging platform to count CD4(+) T cells from a drop of unprocessed whole blood targeting applications at the POC such as HIV management and monitoring. The developed immunochemistry stabilization method can potentially be applied broadly to other diagnostic immunoassays such as viral load measurements, chemotherapy monitoring, and biomarker detection for cancer patients at the POC.en_US
dc.language.isoengen_US
dc.relation.isversionof10.1038/srep21163en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSTRESS-SPECIFIC STABILIZATIONen_US
dc.subjectDRYING-INDUCED DENATURATIONen_US
dc.subjectEMERGING TECHNOLOGIESen_US
dc.subjectPROTEINSen_US
dc.subjectTREHALOSEen_US
dc.subjectCELLen_US
dc.subjectCHEMOTHERAPYen_US
dc.subjectIMMUNOASSAYSen_US
dc.subjectTUBERCULOSISen_US
dc.subjectSEPARATIONen_US
dc.titleEngineering long shelf life multilayer biologically active surfaces on microfluidic devices for point of care applicationsen_US
dc.typearticleen_US
dc.relation.journalSCIENTIFIC REPORTSen_US
dc.identifier.volume6en_US
dc.identifier.wos000370230000001


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