Show simple item record

dc.contributor.authorAysin, Ferhunde
dc.contributor.authorErson-Bensan, Ayse Elif
dc.contributor.authorEyidogan, Fusun
dc.contributor.authorOktem, Huseyin Avni
dc.date.accessioned2019-11-26T08:25:23Z
dc.date.available2019-11-26T08:25:23Z
dc.date.issued2015
dc.identifier.issn1300-008X
dc.identifier.urihttp://journals.tubitak.gov.tr/botany/issues/bot-15-39-5/bot-39-5-2-1405-118.pdf
dc.identifier.urihttp://hdl.handle.net/11727/4238
dc.description.abstractIdentification of vacuolar Na+/H+ antiporters facilitates the basis of salt stress tolerance mechanisms. Na+ accumulation into the vacuole is crucial for the avoidance of cytoplasmic Na+ toxicity. In this study, we show that the introduction of AtNHX1 into tobacco generated more tolerant plants when compared to wild-type plants. Transgenic tobacco plants exhibited higher germination rates in the presence of increasing salt concentrations compared to wild-type plants. In addition, proline levels were higher under salt stress conditions in both the shoots and the roots of transgenics compared to wild-type plants. Increase in malondialdehyde production during lipid peroxidation by salinity was lower in transgenic plants compared to the controls. Being important and newly discovered determiners of plant stress responses, microRNAs (miRNAs) are noncoding small RNAs and essential indicators of plant stress response mechanisms. For further identification of stress responses, the expression levels of growth and abiotic stress-related miRNAs (miR319a, miR319b, miR159b, miR398a, and miR398b) were quantified. miR319a, miR319b, and miR159b expression levels were higher in wildtype plants, and miR319a and miR159b expression was restored in transgenic plants. There was a slight decrease in the expression levels of miR398a and miR398b of wild-type plants. However, the recovery of the miR398a and miR398b expressions was especially significant in transgenic plants. Furthermore, an investigation of miRNAs in transgenic plants can help to understand the stress tolerance mechanisms of the plants.en_US
dc.language.isoengen_US
dc.relation.isversionof10.3906/bot-1405-118en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAtNHX1en_US
dc.subjecttobaccoen_US
dc.subjectsalt stressen_US
dc.subjectmiRNAen_US
dc.titleGenerating salt-tolerant Nicotiana tabacum and identification of stress-responsive miRNAs in transgenicsen_US
dc.typearticleen_US
dc.relation.journalTURKISH JOURNAL OF BOTANYen_US
dc.identifier.volume39en_US
dc.identifier.issue5en_US
dc.identifier.startpage757en_US
dc.identifier.endpage+en_US
dc.identifier.wos000359992200002


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record