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dc.contributor.authorSen, Gamze Tilbe
dc.contributor.authorOzkemahli, Gizem
dc.contributor.authorShahbazi, Reza
dc.contributor.authorErkekoglu, Pinar
dc.contributor.authorUlubayram, Kezban
dc.contributor.authorKocer-Gumusel, Belma
dc.date.accessioned2021-06-16T06:59:46Z
dc.date.available2021-06-16T06:59:46Z
dc.date.issued2020
dc.identifier.issn1091-5818en_US
dc.identifier.urihttp://hdl.handle.net/11727/6020
dc.description.abstractGold nanoparticles (AuNPs) have been widely used in many biological and biomedical applications. In this regard, their surface modification is of paramount importance in order to increase their cellular uptake, delivery capability, and optimize their distribution inside the body. The aim of this study was to examine the effects of AuNPs on cytotoxicity, oxidant/antioxidant parameters, and DNA damage in HepG2 cells and investigate the potential toxic effects of different surface modifications such as polyethylene glycol (PEG) and polyethyleneimine (PEI; molecular weights of 2,000 (low molecular weight [LMW]) and 25,000 (high molecular weight [HMW]). The study groups were determined as AuNPs, PEG-coated AuNPs (AuNPs/PEG), low-molecular weight polyethyleneimine-coated gold nanoparticles (AuNPs/PEI LMW), and high-molecular weight polyethyleneimine-coated gold nanoparticles (AuNPs/PEI HMW). After incubating HepG2 cells with different concentrations of nanoparticles for 24 hours, half maximal inhibitory concentrations (the concentration that kills 50% of the cells) were determined as 166.77, 257.73, and 198.44 mu g/mL for AuNPs, AuNPs/PEG, and AuNPs/PEI LMW groups, respectively. Later, inhibitory concentration 30 (IC30, the concentration that kills 30% of the cells) doses were calculated, and further experiments were performed on cells that were exposed to IC30 doses. Although intracellular reactive oxygen species levels significantly increased in all nanoparticles, AuNPs as well as AuNPs/PEG did not cause any changes in oxidant/antioxidant parameters. However, AuNPs/PEI HMW particularly induced oxidative stress as evidence of alterations in lipid peroxidation and protein oxidation. These results suggest that at IC30 doses, AuNPs do not affect oxidative stress and DNA damage significantly. Polyethylene glycol coating does not have an impact on toxicity, however PEI coating (particularly HMW) can induce oxidative stress.en_US
dc.language.isoengen_US
dc.relation.isversionof10.1177/1091581820927646en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNA damageen_US
dc.subjectgold nanoparticlesen_US
dc.subjectoxidative stressen_US
dc.subjectpolyethylene glycolen_US
dc.subjectpolyethyleneimineen_US
dc.subjectreactive oxygen speciesen_US
dc.subjectsurface modificationen_US
dc.titleThe Effects of Polymer Coating of Gold Nanoparticles on Oxidative Stress and DNA Damageen_US
dc.typearticleen_US
dc.relation.journalINTERNATIONAL JOURNAL OF TOXICOLOGYen_US
dc.identifier.volume39en_US
dc.identifier.issue4en_US
dc.identifier.startpage328en_US
dc.identifier.endpage340en_US
dc.identifier.wos000537487000001en_US
dc.identifier.scopus2-s2.0-85085880887en_US
dc.contributor.pubmedID32483993en_US
dc.contributor.orcID0000-0002-9421-6069en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergien_US


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