Prostaglandin F receptor expression in intrauterine tissues of pregnant rats
View/ Open
Date
2014Author
Anadol, Elvan
Kanca, Halit
Yar, Atiye Seda
Helvacioglu, Fatma
Menevse, Sevda
Calguner, Engin
Erdogan, Deniz
Metadata
Show full item recordAbstract
In this investigation, we studied the expression and localization of rat prostaglandin F (FP) receptor in uterine tissues of rats on gestational Days 10, 15, 18, 20, 21, 21.5 and postpartal Days 1 and 3 using Western blotting analysis, real-time PCR, and immunohistochemistry. A high level of immunoreactivity was observed on gestational Days 20, 21, and 21.5 with the most significant signals found on Day 20. FP receptor protein was expressed starting on gestational Day 15, and a fluctuating unsteady increase was observed until delivery. Uterine FP receptor mRNA levels were low between Days 10 and 18 of gestation (p < 0.05). The transcript level increased significantly on Day 20 and peaked on Day 21.5 just before labor (p < 0.05). There was a positive correlation between FP receptor mRNA expression and serum estradiol levels (rs = 0.78; p < 0.01) along with serum estradiol/progesterone ratios (rs = 0.79; p < 0.01). In summary, we observed an increase FP receptor expression in rat uterus with advancing gestation, a marked elevation of expression at term, and a concominant decrease during the postpartum period. These findings indicate a role for uterine FP receptors in the mediation of uterine contractility at term.
URI
https://synapse.koreamed.org/Synapse/Data/PDFData/0118JVS/jvs-15-125.pdfhttp://hdl.handle.net/11727/4542